Development and Validation of a Novel RP-HPLC Method for Estimation of Losartan Potassium in Dissolution Samples of Immediate and Sustained Release Tablets
Joint Authors
Lalitha, K. G.
Pawar, Harshal A.
Source
Chromatography Research International
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A simple, rapid, selective, and reproducible reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the estimation of Losartan potassium in dissolution samples of Losartan potassium immediate and sustained release tablets.
Analysis was performed on an Agilent, Zorbax Eclipse XDB C18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase consisting of orthophosphoric acid (0.1% v/v)—acetonitrile (55 : 45, v/v) at a flow rate of 1.0 mL/min.
UV detection was performed at 225 nm and the retention time for Losartan was about 2.6 minutes.
The calibration curve was linear (correlation coefficient = 0.999) in the selected range of analyte.
The optimized dissolution conditions include the USP apparatus 2 at a paddle rotation rate of 50 rpm and 900 mL of pH 6.8 phosphate buffer as dissolution medium, at 37.0±0.5∘C.
The method was validated for precision, linearity, specificity, accuracy, limit of quantitation, and ruggedness.
The system suitability parameters, such as theoretical plate, tailing factor and relative standard deviation (RSD) between five standard replicates, were well within the limits.
The stability result shows that the drug is stable in the prescribed dissolution medium.
American Psychological Association (APA)
Pawar, Harshal A.& Lalitha, K. G.. 2014. Development and Validation of a Novel RP-HPLC Method for Estimation of Losartan Potassium in Dissolution Samples of Immediate and Sustained Release Tablets. Chromatography Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494629
Modern Language Association (MLA)
Pawar, Harshal A.& Lalitha, K. G.. Development and Validation of a Novel RP-HPLC Method for Estimation of Losartan Potassium in Dissolution Samples of Immediate and Sustained Release Tablets. Chromatography Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-494629
American Medical Association (AMA)
Pawar, Harshal A.& Lalitha, K. G.. Development and Validation of a Novel RP-HPLC Method for Estimation of Losartan Potassium in Dissolution Samples of Immediate and Sustained Release Tablets. Chromatography Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494629