Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell
Joint Authors
Zhao, Zhujiang
Wu, Qingxiang
Fan, Hong
Zhang, Jianqiong
Cheng, Jian
Qiu, Xuemei
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Promoter hypermethylation mediated by DNA methyltransferases (DNMTs) is the main reason for epigenetic inactivation of tumor suppressor genes (TSGs).
Previous studies showed that DNMT1 and DNMT3B play an important role in CpG island methylation in tumorigenesis.
Little is known about the role of DNMT3A in this process, especially in hepatocellular carcinoma (HCC).
In the present study, increased DNMT3A expression in 3 out of 6 HCC cell lines and 16/25 (64%) HCC tissues implied that DNMT3A is involved in hepatocellular carcinogenesis.
Depletion of DNMT3A in HCC cell line SMMC-7721 inhibited cell proliferation and decreased the colony formation (about 65%).
Microarray data revealed that 153 genes were upregulated in DNMT3A knockdown cells and that almost 71% (109/153) of them contain CpG islands in their 5′ region.
13 of them including PTEN, a crucial tumor suppressor gene in HCC, are genes involved in cell cycle and cell proliferation.
Demethylation of PTEN promoter was observed in DNMT3A-depleted cells implying that DNMT3A silenced PTEN via DNA methylation.
These results provide insights into the mechanisms of DNMT3A to regulate TSGs by an epigenetic approach in HCC.
American Psychological Association (APA)
Zhao, Zhujiang& Wu, Qingxiang& Cheng, Jian& Qiu, Xuemei& Zhang, Jianqiong& Fan, Hong. 2010. Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-494695
Modern Language Association (MLA)
Zhao, Zhujiang…[et al.]. Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-494695
American Medical Association (AMA)
Zhao, Zhujiang& Wu, Qingxiang& Cheng, Jian& Qiu, Xuemei& Zhang, Jianqiong& Fan, Hong. Depletion of DNMT3A Suppressed Cell Proliferation and Restored PTEN in Hepatocellular Carcinoma Cell. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-494695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494695