Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis
Joint Authors
Wang, Xiuli
Cui, Fuai
Cui, Quanjun
Dighe, Abhijit S.
Keller, Tom
Balian, Gary
Seamon, Jesse
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The combined delivery of mesenchymal stem cells (MSCs), vascular endothelial growth factor (VEGF), and bone morphogenetic protein (BMP) to sites of bone injury results in enhanced repair compared to the administration of a single factor or a combination of two factors.
Based on these findings, we hypothesized that coexpression of VEGF and BMP-6 genes would enhance the osteoblastic differentiation of rat bone-marrow-derived stem cells (rMSCs) and osteogenesis by comparison to rMSCs that do not express VEGF and BMP-6.
We prepared a GFP tagged adenovirus vector (Ad-VEGF+BMP-6) that contained DNA encoding the hVEGF and hBMP-6 genes.
rMSCs were transduced with the virus, and the successful transduction was confirmed by green fluorescence and by production of VEGF and BMP-6 proteins.
The cells were cultured to assess osteoblastic differentiation or administered in the Fischer 344 rats to assess bone formation.
Mineralization of rMSCs transduced with Ad-VEGF+BMP-6 was significantly enhanced over the nontransduced rMSCs.
Only transduced rMSCs could induce osteogenesis in vivo, whereas Ad-VEGF+BMP-6 or nontransduced rMSCs alone did not induce osteogenesis.
The data suggests that the combined delivery of MSCs, VEGF, and BMP-6 is an attractive option for bone repair therapy.
American Psychological Association (APA)
Seamon, Jesse& Wang, Xiuli& Cui, Fuai& Keller, Tom& Dighe, Abhijit S.& Balian, Gary…[et al.]. 2013. Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis. Bone Marrow Research،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-494697
Modern Language Association (MLA)
Seamon, Jesse…[et al.]. Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis. Bone Marrow Research No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-494697
American Medical Association (AMA)
Seamon, Jesse& Wang, Xiuli& Cui, Fuai& Keller, Tom& Dighe, Abhijit S.& Balian, Gary…[et al.]. Adenoviral Delivery of the VEGF and BMP-6 Genes to Rat Mesenchymal Stem Cells Potentiates Osteogenesis. Bone Marrow Research. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-494697
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494697