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In Vitro Antibacterial and Time-Kill Evaluation of the Erythrina caffra Thunb. Extract against Bacteria Associated with Diarrhoea
Joint Authors
Afolayan, Anthony Jide
Olajuyigbe, Olufunmiso Olusola
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The antibacterial activities of stem bark ethanolic extract of Erythrina caffra Thunb.
against bacteria in diarrhoea was determined in vitro by the agar diffusion and dilution, macrobroth dilution, and time-kill assay methods.
The result showed that the extract produced inhibition zones ranging between 15±1.0 mm and 23±1.0 mm, and the bacteria were susceptible at concentrations ranging between ≤100 and ≤1000 μg/mL.
While the MICs of the extract ranged between 39.1 and 625 μg/mL, and the MBCs ranged between 78.1 and 625 μg/mL, the MICs of Micrococcus luteus, Proteus vulgaris CSIR 0030, Enterococcus faecalis KZN, and Staphylococcus aureus OK3 were less than 100 μg/mL, and the mechanisms of antibiosis indicated that the crude ethanolic extract was highly bactericidal against the entire test bacteria isolates.
In the time-kill assay, the average log reduction of the viable cell count ranged between 0.916log 10 and 1.851log 10 cfu/mL on incubating the bacteria for 4 h at the MICs, while the reduction ranged between 0.183log 10 and 1.105log 10 cfu/mL after 8 h of incubation.
Incubating the bacteria for 4 h at 2 × MICs resulted in the reduction of the viable cell count to between −0.264log 10 and 0.961log 10 cfu/mL, while the average log reduction ranged between −3.968log 10 and −0.425log 10 cfu/mL after 8 h of incubation with Micrococcus luteus, Proteus vulgaris CSIR 0030, and Staphylococcus aureus OK3 being the most highly affected bacteria.
The result showed that the extract exhibited broader-spectrum antibacterial activity and justifies the use of Erythrina caffra in the folkloric medicine for treating gastrointestinal infections in South Africa.
American Psychological Association (APA)
Olajuyigbe, Olufunmiso Olusola& Afolayan, Anthony Jide. 2012. In Vitro Antibacterial and Time-Kill Evaluation of the Erythrina caffra Thunb. Extract against Bacteria Associated with Diarrhoea. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-494755
Modern Language Association (MLA)
Olajuyigbe, Olufunmiso Olusola& Afolayan, Anthony Jide. In Vitro Antibacterial and Time-Kill Evaluation of the Erythrina caffra Thunb. Extract against Bacteria Associated with Diarrhoea. The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-494755
American Medical Association (AMA)
Olajuyigbe, Olufunmiso Olusola& Afolayan, Anthony Jide. In Vitro Antibacterial and Time-Kill Evaluation of the Erythrina caffra Thunb. Extract against Bacteria Associated with Diarrhoea. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-494755
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494755