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Molecular Approach for Tracing Dissemination Routes of Shiga Toxin-Producing Escherichia coli O157 in Bovine Offal at Slaughter
Joint Authors
Igimi, Shizunobu
Masuda, Kazuya
Asakura, Hiroshi
Yamamoto, Shigeki
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-30
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Bovine offal is currently recognized as one of the sources of human Shiga toxin-producing Escherichia coli (STEC) infection in Japan.
Here, the prevalence and genetic characterization of STEC O157 in bovine feces, offal, and carcasses at slaughtering were examined between July and October in 2006.
STEC O157 was detected in 31 of 301 cattle feces (10.3%) delivered from 120 farms.
Simultaneously, 60 bovine-originated offal (tongue, liver, and omasum) and carcasses were randomly selected and the detection of O157 STEC was examined as well.
STEC O157 was isolated from 4 tongues (6.7%), 1 liver (1.7%), 3 omasa (5.0%), and 2 carcasses (3.3%), respectively.
All the O157 isolates were positive for eae and hlyA genes, and 37 of 41 isolates (90.2%) exhibited stx2c genotype.
PFGE analysis revealed the identical macrogenotypes of 4-tongue- and 1-liver-originated isolates and among 2 fecal isolates from animals slaughtered consecutively.
Considering their continuous detection according to the slaughtering order, we concluded that these distributions of O157 in bovine offal and feces might be due to cross-contamination at (pre)slaughter.
Our data thus reposes implication of better sanitary control in diapedesis from both upper and lower sites to prevent spread of this pathogen to bovine offal at slaughtering.
American Psychological Association (APA)
Asakura, Hiroshi& Masuda, Kazuya& Yamamoto, Shigeki& Igimi, Shizunobu. 2014. Molecular Approach for Tracing Dissemination Routes of Shiga Toxin-Producing Escherichia coli O157 in Bovine Offal at Slaughter. BioMed Research International،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-494825
Modern Language Association (MLA)
Asakura, Hiroshi…[et al.]. Molecular Approach for Tracing Dissemination Routes of Shiga Toxin-Producing Escherichia coli O157 in Bovine Offal at Slaughter. BioMed Research International No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-494825
American Medical Association (AMA)
Asakura, Hiroshi& Masuda, Kazuya& Yamamoto, Shigeki& Igimi, Shizunobu. Molecular Approach for Tracing Dissemination Routes of Shiga Toxin-Producing Escherichia coli O157 in Bovine Offal at Slaughter. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-494825
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494825