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Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics
Joint Authors
Kondo, Tadashi
Murakami, Yasufumi
Saito, Yumi
Hagiwara, Tatsuo
Nakamura, Yukiko
Tomonaga, Takeshi
Source
International Journal of Proteomics
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The intact plasma proteome is of great interest in biomarker studies because intact proteins reflect posttranslational protein processing such as phosphorylation that may correspond to disease status.
We examined the utility of a solid-phase hexapeptide ligand library in combination with conventional plasma proteomics modalities for comprehensive profiling of intact plasma proteins.
Plasma proteins were sequentially fractionated using depletion columns for albumin and immunoglobulin, and separated using an anion-exchange column.
Proteins in each fraction were treated with a solid-phase hexapeptide ligand library and compared to those without treatment.
Two-dimensional difference gel electrophoresis demonstrated an increased number of protein spots in the treated samples.
Mass spectrometric studies of these protein spots with unique intensity in the treated samples resulted in the identification of high- and medium-abundance proteins.
Our results demonstrated the possible utility of a solid-phase hexapeptide ligand library to reveal greater number of intact plasma proteins.
The characteristics of proteins with unique affinity to the library remain to be clarified by more extensive mass spectrometric protein identification, and optimized protocols should be established for large-scale plasma biomarker studies.
American Psychological Association (APA)
Hagiwara, Tatsuo& Saito, Yumi& Nakamura, Yukiko& Tomonaga, Takeshi& Murakami, Yasufumi& Kondo, Tadashi. 2011. Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-494873
Modern Language Association (MLA)
Hagiwara, Tatsuo…[et al.]. Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics. International Journal of Proteomics No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-494873
American Medical Association (AMA)
Hagiwara, Tatsuo& Saito, Yumi& Nakamura, Yukiko& Tomonaga, Takeshi& Murakami, Yasufumi& Kondo, Tadashi. Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-494873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494873