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Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival
Joint Authors
Navarro, Sonia
Mitsuhashi, Noboru
Kearns-Jonker, Mary
Evans, Jacqueline M.
Doki, Tomoko
Stewart, John M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Allograft survival can be prolonged by overexpression of cytoprotective genes such as heme oxygenase-1 (HO-1).
Modifications in vector design and delivery have provided new opportunities to safely and effectively administer HO-1 into the heart prior to transplantation to improve long-term graft outcome.
Methods.
HO-1 was delivered to the donor heart using an adeno-associated virus vector (AAV) with a pseudotype 6 capsid and vascular endothelial growth factor (VEGF) to enhance myocardial tropism and microvascular permeability.
Survival of mouse cardiac allografts, fully or partially mismatched at the MHC, was determined with and without cyclosporine A.
Intragraft cytokine gene expression was examined by PCR.
Results.
The use of AAV6 to deliver HO-1 to the donor heart, combined with immunosuppression, prolonged allograft survival by 55.3% when donor and recipient were completely mismatched at the MHC and by 94.6% if partially mismatched.
The combination of gene therapy and immunosuppression was more beneficial than treatment with either AAV6-HO-1 or CsA alone.
IL-17a, b, e and f were induced in the heart at rejection.
Conclusions.
Pretreatment of cardiac allografts with AAV6-HO-1 plus cyclosporine A prolonged graft survival.
HO-1 gene therapy represents a beneficial adjunct to immunosuppressive therapy in cardiac transplantation.
American Psychological Association (APA)
Evans, Jacqueline M.& Navarro, Sonia& Doki, Tomoko& Stewart, John M.& Mitsuhashi, Noboru& Kearns-Jonker, Mary. 2012. Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival. Journal of Transplantation،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-494960
Modern Language Association (MLA)
Evans, Jacqueline M.…[et al.]. Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival. Journal of Transplantation No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-494960
American Medical Association (AMA)
Evans, Jacqueline M.& Navarro, Sonia& Doki, Tomoko& Stewart, John M.& Mitsuhashi, Noboru& Kearns-Jonker, Mary. Gene Transfer of Heme Oxygenase-1 Using an Adeno-Associated Virus Serotype 6 Vector Prolongs Cardiac Allograft Survival. Journal of Transplantation. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-494960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494960