![](/images/graphics-bg.png)
Insights into the Alteration of Osteoblast Mechanical Properties upon Adhesion on Chitosan
Joint Authors
Athanassiou, George M.
Moutzouri, Antonia G.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Cell adhesion on substrates is accompanied by significant changes in shape and cytoskeleton organization, which affect subsequent cellular and tissue responses, determining the long-term success of an implant.
Alterations in osteoblast stiffness upon adhesion on orthopaedic implants with different surface chemical composition and topography are, thus, of central interest in the field of bone implant research.
This work aimed to study the mechanical response of osteoblasts upon adhesion on chitosan-coated glass surfaces and to investigate possible correlations with the level of adhesion, spreading, and cytoskeleton reorganization.
Using the micropipette aspiration technique, the osteoblast elastic modulus was found higher on chitosan-coated than on uncoated control substrates, and it was found to increase in the course of spreading for both substrates.
The cell-surface contact area was measured throughout several time points of adhesion to quantify cell spreading kinetics.
Significant differences were found between chitosan and control surfaces regarding the response of cell spreading, while both groups displayed a sigmoidal kinetical behavior with an initially elevated spreading rate which stabilizes in the second hour of attachment.
Actin filament structural changes were confirmed after observation with confocal microscope.
Biomaterial surface modification can enhance osteoblast mechanical response and induce favorable structural organization for the implant integration.
American Psychological Association (APA)
Moutzouri, Antonia G.& Athanassiou, George M.. 2014. Insights into the Alteration of Osteoblast Mechanical Properties upon Adhesion on Chitosan. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494966
Modern Language Association (MLA)
Moutzouri, Antonia G.& Athanassiou, George M.. Insights into the Alteration of Osteoblast Mechanical Properties upon Adhesion on Chitosan. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-494966
American Medical Association (AMA)
Moutzouri, Antonia G.& Athanassiou, George M.. Insights into the Alteration of Osteoblast Mechanical Properties upon Adhesion on Chitosan. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-494966
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494966