Rheumatoid Factor and Its Interference with Cytokine Measurements : Problems and Solutions
Joint Authors
Falbe Wätjen, Inger
Bartels, Else Marie
Bliddal, Henning
Danneskiold-Samsøe, Bente
Littrup Andersen, Eva
Ribel-Madsen, Søren
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Use of cytokines as biomarkers for disease is getting more widespread.
Cytokines are conveniently determined by immunoassay, but interference from present antibodies is known to cause problems.
In rheumatoid arthritis (RA), interference of rheumatoid factor (RF) may be problematic.
RF covers a group of autoantibodies from immunoglobulin subclasses and is present in 65–80% of RA patients.
Partly removal of RF is possible by precipitation.
This study aims at determining the effects of presence of RF in blood and synovial fluid on cytokine measurements in samples from RA patients and finding possible solutions for recognized problems.
IL-1β, IL-4, IL-6, and IL-8 were determined with multiplex immunoassays (MIA) in samples from RA patients prior to and after polyethylene glycol (PEG 6000) precipitation.
Presence of RF does interfere with MIA.
PEG 6000 precipitation abolishes this RF interference.
We recommend PEG precipitation for all immunoassay measurements of plasma samples from RA patients.
American Psychological Association (APA)
Bartels, Else Marie& Falbe Wätjen, Inger& Littrup Andersen, Eva& Danneskiold-Samsøe, Bente& Bliddal, Henning& Ribel-Madsen, Søren. 2011. Rheumatoid Factor and Its Interference with Cytokine Measurements : Problems and Solutions. Arthritis،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-495000
Modern Language Association (MLA)
Bartels, Else Marie…[et al.]. Rheumatoid Factor and Its Interference with Cytokine Measurements : Problems and Solutions. Arthritis No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-495000
American Medical Association (AMA)
Bartels, Else Marie& Falbe Wätjen, Inger& Littrup Andersen, Eva& Danneskiold-Samsøe, Bente& Bliddal, Henning& Ribel-Madsen, Søren. Rheumatoid Factor and Its Interference with Cytokine Measurements : Problems and Solutions. Arthritis. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-495000
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495000