Phylogenetic Relationships of Tetraploid AB-Genome Avena Species Evaluated by Means of Cytogenetic (C-Banding and FISH)‎ and RAPD Analyses

Abstract EN

Tetraploid oat species Avena abyssinica, A.

vaviloviana, A.

barbata, and A.

agadiriana were studied using C-banding technique, in situ hybridization with the 45S and 5S rDNA probes, and RAPD analysis in comparison with the diploid species carrying different types of the A-genome (A.

wiestii, As; A.

longiglumis, Al; A.

canariensis, Ac; A.

damascena, Ad, A.

prostrata, Ap).

The investigation confirmed that all four tetraploids belong to the same AB-genome group; however A.

agadiriana occupies distinct position among others.

The C-banding, FISH, and RAPD analyses showed that Avena abyssinica, A.

vaviloviana, and A.

barbata are very similar; most probably they originated from a common tetraploid ancestor as a result of minor translocations and alterations of C-banding polymorphism system.

AB-genome species are closely related with the A-genome diploids, and an As-genome species may be regarded as the most probable donor of their A-genome.

Although their second diploid progenitor has not been identified, it seems unlikely that it belongs to the As-genome group.

The exact diploid progenitors of A.

agadiriana have not been determined; however our results suggest that at least one of them could be related to A.

damascena.