Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-Tripeptide Transporter, hPEPT1
Joint Authors
Søndergaard, Helle Bach
Nielsen, Carsten Uhd
Brodin, Birger
Source
International Journal of Peptides
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The human H+-coupled di-/tripeptide transporter (hPEPT1) mediates intestinal absorption of dietary di- and tripeptides, as well as several peptidomimetic drug compounds.
The aim of the present study was to investigate the possible role of the hPEPT1 variant hPEPT1-RF in hPEPT1 regulation.
However, the proposed hPEPT1-RF mRNA sequence could not be detected in Caco-2 cells or in human intestinal samples.
Instead, a new sequence variant, hPEPT1-RFI, was found, which is almost identical to the proposed hPEPT1-RF, except for two nucleotide insertions and one deletion that resulted in a changed open reading frame as compared to hPEPT1-RF.
In vitro translation analysis showed that hPEPT1-RFI was not translated.
In conclusion, the existence of hPEPT1-RF could not be confirmed; furthermore, the identified sequence variant, hPEPT1-RFI, does not appear to be translated and is therefore unlikely to have a regulatory effect on hPEPT1 transport activity.
American Psychological Association (APA)
Søndergaard, Helle Bach& Nielsen, Carsten Uhd& Brodin, Birger. 2012. Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-Tripeptide Transporter, hPEPT1. International Journal of Peptides،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-495224
Modern Language Association (MLA)
Søndergaard, Helle Bach…[et al.]. Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-Tripeptide Transporter, hPEPT1. International Journal of Peptides No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-495224
American Medical Association (AMA)
Søndergaard, Helle Bach& Nielsen, Carsten Uhd& Brodin, Birger. Identification and Characterization of a Novel Nontranslated Sequence Variant of the Human Intestinal Di-Tripeptide Transporter, hPEPT1. International Journal of Peptides. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-495224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495224