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A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells
Joint Authors
Baud, Véronique
Reis, Rosana I.
Rodrigues-Ferreira, Sylvie
Costa-Neto, Claudio M.
Cormier, Françoise
Morel, Marina
Nahmias, Clara
Source
International Journal of Peptides
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-06
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Recent studies have highlighted the AT1 receptor as a potential therapeutic target in breast cancer, while the role of the AT2 subtype in this disease has remained largely neglected.
The present study describes the generation and characterization of a new cellular model of human invasive breast cancer cells (D3H2LN-AT2) stably expressing high levels of Flag-tagged human AT2 receptor (Flag-hAT2).
These cells exhibit high-affinity binding sites for AngII, and total binding can be displaced by the AT2-selective antagonist PD123319 but not by the AT1-selective antagonist losartan.
Of interest, high levels of expression of luciferase and green fluorescent protein make these cells suitable for bioluminescence and fluorescence studies in vitro and in vivo.
We provide here a novel tool to investigate the AT2 receptor functions in breast cancer cells, independently of AT1 receptor activation.
American Psychological Association (APA)
Rodrigues-Ferreira, Sylvie& Morel, Marina& Reis, Rosana I.& Cormier, Françoise& Baud, Véronique& Costa-Neto, Claudio M.…[et al.]. 2011. A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells. International Journal of Peptides،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495291
Modern Language Association (MLA)
Rodrigues-Ferreira, Sylvie…[et al.]. A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells. International Journal of Peptides No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-495291
American Medical Association (AMA)
Rodrigues-Ferreira, Sylvie& Morel, Marina& Reis, Rosana I.& Cormier, Françoise& Baud, Véronique& Costa-Neto, Claudio M.…[et al.]. A Novel Cellular Model to Study Angiotensin II AT2 Receptor Function in Breast Cancer Cells. International Journal of Peptides. 2011. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-495291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495291