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Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools
Joint Authors
Pollok-Kopp, B.
Schlaf, G.
Altermann, W.
Schabel, E.
Source
Case Reports in Transplantation
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Allografting patients with human leukocyte antigens (HLA) which are recognized by preformed antibodies constitutes the main cause for hyper-acute or acute rejections.
In order to select recipients without these donor-specific antibodies, the complement-dependent cytotoxicity crossmatch (CDC-CM) assay was developed as a standard procedure about forty years ago.
The negative outcome of pretransplant crossmatching represents the most important requirement for a successful kidney graft survival.
The artificially positive outcomes of CDC-based crossmatches due to the underlying disease Systemic Lupus Erythematosus (SLE), however, may lead to the unjustified refusal of adequate kidney grafts.
Two prospective female recipients destined for a living as well as for a cadaver kidney donation, respectively, exhibited positive CDC-based crossmatch outcomes although for both patients no historical immunizing events were known.
Furthermore, solid phase-based screening or antibody differentiation analyses never led to positive results.
Immediate reruns of the CDC-based crossmatch assays using the alternative antibody monitoring system (AMS-)crossmatch ELISA resulted in unequivocally negative outcomes.
Consequently both transplantations were performed without any immunological complications for the hitherto follow-up time of 25 and 28 months, respectively.
We here show two case reports demonstrating an alternative methodical approach to circumvent CDC-based artefacts and point to the urgent need to substitute the CDC-based crossmatch procedure at least for special groups of patients.
American Psychological Association (APA)
Schlaf, G.& Pollok-Kopp, B.& Schabel, E.& Altermann, W.. 2013. Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools. Case Reports in Transplantation،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-495412
Modern Language Association (MLA)
Schlaf, G.…[et al.]. Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools. Case Reports in Transplantation No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-495412
American Medical Association (AMA)
Schlaf, G.& Pollok-Kopp, B.& Schabel, E.& Altermann, W.. Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools. Case Reports in Transplantation. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-495412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495412