Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung
Joint Authors
Knuefermann, Pascal
Meyer, Rainer
Ehrentraut, H.
Ehrentraut, S.
Schwederski, Markus
Velten, M.
Baumgarten, Georg
Grohé, C.
Boehm, Olaf
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
To determine whether systemically administered TLR ligands differentially modulate pulmonary inflammation.
Methods.
Equipotent doses of LPS (20 mg/kg), CpG-ODN (1668-thioat 1 nmol/g), or LTA (15 mg/kg) were determined via TNF activity assay.
C57BL/6 mice were challenged intraperitoneally.
Pulmonary NFκB activation (2 h) and gene expression/activity of key inflammatory mediators (4 h) were monitored.
Results.
All TLR ligands induced NFκB.
LPS increased the expression of TLR2, 6, and the cytokines IL-1αβ, TNF-α, IL-6, and IL-12p35/p40, CpG-ODN raised TLR6, TNF-α, and IL12p40.
LTA had no effect.
Additionally, LPS increased the chemokines MIP-1α/β, MIP-2, TCA-3, eotaxin, and IP-10, while CpG-ODN and LTA did not.
Myeloperoxidase activity was highest after LPS stimulation.
MMP1, 3, 8, and 9 were upregulated by LPS, MMP2, 8 by CpG-ODN and MMP2 and 9 by LTA.
TIMPs were induced only by LPS.
MMP-2/-9 induction correlated with their zymographic activities.
Conclusion.
Pulmonary susceptibility to systemic inflammation was highest after LPS, intermediate after CpG-ODN, and lowest after LTA challenge.
American Psychological Association (APA)
Ehrentraut, H.& Meyer, Rainer& Schwederski, Markus& Ehrentraut, S.& Velten, M.& Grohé, C.…[et al.]. 2011. Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung. Mediators of Inflammation،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-495428
Modern Language Association (MLA)
Ehrentraut, H.…[et al.]. Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung. Mediators of Inflammation No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-495428
American Medical Association (AMA)
Ehrentraut, H.& Meyer, Rainer& Schwederski, Markus& Ehrentraut, S.& Velten, M.& Grohé, C.…[et al.]. Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung. Mediators of Inflammation. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-495428
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495428