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Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles
Joint Authors
Sassi, Zina
Abdelghani, Adnane
Bessueille, Francois
Gallouz, Asma
Mohamed Hassen, Walid
Hafaiedh, Imène
Jaffrezic-Renault, Nicole
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This work has explored the development of impedimetric immunosensors based on magnetic iron nanoparticles (IrNP) functionalized with streptavidin to which a biotinylated FAB part of the antibody has been bound using a biotin-streptavidin interaction.
SPR analysis shows a deviation on the measured (angle) during antigen-antibody recognition whereas label free detection using by EIS allows us to monitor variation of polarization resistance.
Before detection, layers were analyzed by FTIR and AFM.
Compared to immobilization of antibody on bare gold surface using aminodecanethiol SAM, antibody immobilization on nanoparticles permitted to reach lower detection limit: 500 pg/ml instead of 1 ng/ml to in the case of EIS and 300 ng/ml instead of 4.5 μg/ml in the case of SPR.
Thus, it permitted to improve the sensitivity: from 257.3 Ω⋅cm2⋅μg−1⋅ml to 1871 Ω⋅cm2⋅μg−1⋅ml in the case of EIS and from 0.003°μg−1⋅ml to 0.094°μg−1⋅ml in the case of SPR.
American Psychological Association (APA)
Hafaiedh, Imène& Gallouz, Asma& Mohamed Hassen, Walid& Abdelghani, Adnane& Sassi, Zina& Bessueille, Francois…[et al.]. 2009. Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles. Journal of Sensors،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-495432
Modern Language Association (MLA)
Hafaiedh, Imène…[et al.]. Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles. Journal of Sensors No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-495432
American Medical Association (AMA)
Hafaiedh, Imène& Gallouz, Asma& Mohamed Hassen, Walid& Abdelghani, Adnane& Sassi, Zina& Bessueille, Francois…[et al.]. Sensitivity Improvement of an Impedimetric Immunosensor Using Functionalized Iron Oxide Nanoparticles. Journal of Sensors. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-495432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495432