NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy
Joint Authors
Tarr, Joanna M.
Mustapha, Nik M.
Kohner, Eva M.
Chibber, Rakesh
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objectives.
Using apocynin (inhibitor of NADPH oxidase), and Mitoquinol 10 nitrate (MitoQ; mitochondrial-targeted antioxidant), we addressed the importance of mitochondria versus NADPH oxidase-derived ROS in glucose-induced apoptosis of pericytes.
Methods.
NADPH oxidase was localised using Western blot analysis and cytochrome C reduction assay.
Apoptosis was detected by measuring caspase-3 activity.
Intracellular glucose concentration, ROS formation and Nε-(carboxymethyl) lysine (CML) content were measured using Amplex Red assay kit, dihydroethidium (DHE), and competitive immunoabsorbant enzyme-linked assay (ELISA), respectively.
Results.
NADPH oxidase was localised in the cytoplasm of pericytes suggesting ROS production within intracellular compartments.
High glucose (25 mM) significantly increased apoptosis, intracellular glucose concentration, and CML content.
Apoptosis was associated with increased gp91phox expression, activity of NADPH oxidase, and intracellular ROS production.
Apocynin and not MitoQ significantly blunted the generation of ROS, formation of intracellular CML and apoptosis.
Conclusions.
NADPH oxidase and not mitochondria-derived ROS is responsible for the accelerated apoptosis of pericytes in diabetic retinopathy.
American Psychological Association (APA)
Mustapha, Nik M.& Tarr, Joanna M.& Kohner, Eva M.& Chibber, Rakesh. 2010. NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy. Journal of Ophthalmology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-495467
Modern Language Association (MLA)
Mustapha, Nik M.…[et al.]. NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy. Journal of Ophthalmology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-495467
American Medical Association (AMA)
Mustapha, Nik M.& Tarr, Joanna M.& Kohner, Eva M.& Chibber, Rakesh. NADPH Oxidase versus Mitochondria-Derived ROS in Glucose-Induced Apoptosis of Pericytes in Early Diabetic Retinopathy. Journal of Ophthalmology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-495467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495467