![](/images/graphics-bg.png)
Optimum Combination of Insulin-Transferrin-Selenium and Fetal Bovine Serum for Culture of Rabbit Articular Chondrocytes in Three-Dimensional Alginate Scaffolds
Joint Authors
Song, Hong
Zhang, Lanlan
Zhao, Xiaojun
Source
International Journal of Cell Biology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-05-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Fetal bovine serum (FBS) has been reported to affect chondrocyte biosynthesis in monolayer culture.
Insulin-Transferrin-Selenium (ITS) was investigated as a partial replacement for FBS during in vitro culture of rabbit articular chondrocytes in three-dimensional alginate scaffold.
Chondrocyte-seeded alginate hydrogels were cultured in Dulbecco's modified Eagle's medium plus 10% FBS, 1% ITS plus 2% FBS, 1% ITS plus 4% FBS, or 1% ITS plus 8% FBS.
At designed time point, the Chondrocyte-seeded alginate hydrogels were harvested and evaluated with histological staining, immunohistochemistry, and quantitative gene expression analysis.
Viable cell density and cell division were also evaluated.
Chondrocytes biosynthesis and cell division in 1% ITS with 2% FBS medium were similar to that in medium added with 10% FBS.
For a total culture of 3 weeks, phenotypic gene expression in chondrocyte-seeded hydrogels was maintained at high levels in medium with 1% ITS plus 2% FBS, while it was decreased to varying degrees in the other groups.
In conclusion, with 1% ITS, medium with 2% FBS could promote chondrocyte biosynthesis and cell division, and prevented cell dedifferentiation in three-dimensional alginate scaffolds.
American Psychological Association (APA)
Zhang, Lanlan& Song, Hong& Zhao, Xiaojun. 2009. Optimum Combination of Insulin-Transferrin-Selenium and Fetal Bovine Serum for Culture of Rabbit Articular Chondrocytes in Three-Dimensional Alginate Scaffolds. International Journal of Cell Biology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-495469
Modern Language Association (MLA)
Zhang, Lanlan…[et al.]. Optimum Combination of Insulin-Transferrin-Selenium and Fetal Bovine Serum for Culture of Rabbit Articular Chondrocytes in Three-Dimensional Alginate Scaffolds. International Journal of Cell Biology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-495469
American Medical Association (AMA)
Zhang, Lanlan& Song, Hong& Zhao, Xiaojun. Optimum Combination of Insulin-Transferrin-Selenium and Fetal Bovine Serum for Culture of Rabbit Articular Chondrocytes in Three-Dimensional Alginate Scaffolds. International Journal of Cell Biology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-495469
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495469