Expression of TLR4-MyD88 and NF-κB in the Iris during Endotoxin-Induced Uveitis
Joint Authors
Hu, Xiaofeng
Li, Shang
Chen, Wei
Xu, Yingzhi
Wang, Jing
Lu, Hong
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
To observe the expression of Toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B p65 (NF-κB p65) in iris tissue during endotoxin-induced uveitis (EIU) and evaluate the significance of these factors in uveitis.
Methods.
Wistar rats were randomly divided into 5 groups (0 h, 12 h, 24 h, 48 h, and 72 h, n = 10/group).
Animal model of acute anterior uveitis was established by a hind footpad injection of 200 μg Cholera vibrio LPS.
Expression of TLR4, MyD88, and NF-κB p65 in iris ciliary body tissue was detected through immunohistochemical staining.
Results.
Expression of TLR4 was not detected in normal iris-ciliary body complex, TLR4 positive cells with round morphology appeared in the iris stroma 12 hours after injection, significantly increased (P<.001) 48 hours after injection, and decreased gradually 72 hours after injection.
Expression of MyD88 and NF-κB p65 is consistent with the change of the TLR4.
Conclusions.
The increased expression of TLR4 and its downstream signal transduction moleculesMyD88, NF-κB p65 indicate the potential role of pathway in the pathogenesis of acute anterior uveitis (AAU).
American Psychological Association (APA)
Li, Shang& Lu, Hong& Hu, Xiaofeng& Chen, Wei& Xu, Yingzhi& Wang, Jing. 2010. Expression of TLR4-MyD88 and NF-κB in the Iris during Endotoxin-Induced Uveitis. Mediators of Inflammation،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-495556
Modern Language Association (MLA)
Li, Shang…[et al.]. Expression of TLR4-MyD88 and NF-κB in the Iris during Endotoxin-Induced Uveitis. Mediators of Inflammation No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-495556
American Medical Association (AMA)
Li, Shang& Lu, Hong& Hu, Xiaofeng& Chen, Wei& Xu, Yingzhi& Wang, Jing. Expression of TLR4-MyD88 and NF-κB in the Iris during Endotoxin-Induced Uveitis. Mediators of Inflammation. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-495556
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495556