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FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for In Vitro Contamination
Joint Authors
Ferrari, Patrícia Helena
Nakamura, Vitor Cesar
Gavini, Giulio
Cai, Silvana
Kataoka, Simony Hidee Hamoy
Source
International Journal of Dentistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Objective.
To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation.
Methods.
Twenty-five human single-rooted teeth were used.
The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (n=5), according to the preparation methods employed—control group: no solutions applied; group 1: cement removal and irrigation with 5.25% NaOCl +17 % EDTA for 4 minutes each; group 2: 17% EDTA+2.5% NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17% EDTA + 5.25% NaOCl + phosphate buffer solution + distilled water (10 minutes ultrasonic); group 4: 17% EDTA+5.25% NaOCl (3 minutes ultrasonic bath).
Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification.
Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test.
Results.
In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4.
Conclusion.
The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.
American Psychological Association (APA)
Nakamura, Vitor Cesar& Kataoka, Simony Hidee Hamoy& Gavini, Giulio& Ferrari, Patrícia Helena& Cai, Silvana. 2012. FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for In Vitro Contamination. International Journal of Dentistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-495583
Modern Language Association (MLA)
Nakamura, Vitor Cesar…[et al.]. FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for In Vitro Contamination. International Journal of Dentistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-495583
American Medical Association (AMA)
Nakamura, Vitor Cesar& Kataoka, Simony Hidee Hamoy& Gavini, Giulio& Ferrari, Patrícia Helena& Cai, Silvana. FE-SEM Evaluation of Dental Specimens Prepared by Different Methods for In Vitro Contamination. International Journal of Dentistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-495583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495583