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Pretreatment with Pancaspase Inhibitor (Z-VAD-FMK) Delays but Does Not Prevent Intraperitoneal Heat-Killed Group B Streptococcus-Induced Preterm Delivery in a Pregnant Mouse Model
Joint Authors
Ilievski, Vladimir
Moffatt-Blue, Chantelle
Equils, Ozlem
Simmons, Charles F.
Hirsch, Emmet
Ishikawa, Tomo-o
Source
Infectious Diseases in Obstetrics and Gynecology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Caspases and apoptosis are thought to play a role in infection-associated preterm-delivery.
We have shown that in vitro treatment with pancaspase inhibitor Z-VAD-FMK protects trophoblasts from microbial antigen-induced apoptosis.
Objective.
To examine whether in vivo administration of Z-VAD-FMK would prevent infection-induced preterm-delivery.
Methods.
We injected 14.5 day-pregnant-mice with heat-killed group B streptococcus (HK-GBS).
Apoptosis within placentas and membranes was assessed by TUNEL staining.
Calpain expression and caspase-3 activation were assessed by immunohistochemistry.
Preterm-delivery was defined as expulsion of a fetus within 48 hours after injection.
Results.
Intrauterine (i.u.) or intraperitoneal (i.p.) HK-GBS injection led to preterm-delivery and induced apoptosis in placentas and membranes at 14 hours.
The expression of calpain, a caspase-independent inducer of apoptosis, was increased in placenta.
Treatment with the specific caspase inhibitor Z-VAD-FMK (i.p.) prior to HK-GBS (i.p.) delayed but did not prevent preterm-delivery.
Conclusion.
Caspase-dependent apoptosis appears to play a role in the timing but not the occurrence of GBS-induced preterm delivery in the mouse.
American Psychological Association (APA)
Equils, Ozlem& Moffatt-Blue, Chantelle& Ishikawa, Tomo-o& Simmons, Charles F.& Ilievski, Vladimir& Hirsch, Emmet. 2009. Pretreatment with Pancaspase Inhibitor (Z-VAD-FMK) Delays but Does Not Prevent Intraperitoneal Heat-Killed Group B Streptococcus-Induced Preterm Delivery in a Pregnant Mouse Model. Infectious Diseases in Obstetrics and Gynecology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-495674
Modern Language Association (MLA)
Equils, Ozlem…[et al.]. Pretreatment with Pancaspase Inhibitor (Z-VAD-FMK) Delays but Does Not Prevent Intraperitoneal Heat-Killed Group B Streptococcus-Induced Preterm Delivery in a Pregnant Mouse Model. Infectious Diseases in Obstetrics and Gynecology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-495674
American Medical Association (AMA)
Equils, Ozlem& Moffatt-Blue, Chantelle& Ishikawa, Tomo-o& Simmons, Charles F.& Ilievski, Vladimir& Hirsch, Emmet. Pretreatment with Pancaspase Inhibitor (Z-VAD-FMK) Delays but Does Not Prevent Intraperitoneal Heat-Killed Group B Streptococcus-Induced Preterm Delivery in a Pregnant Mouse Model. Infectious Diseases in Obstetrics and Gynecology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-495674
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495674