Apoptotic Cell Death and Inhibition of Wntβ-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus
Joint Authors
Lee, Liang-Ming
Chuang, Shuang-En
Yeh, Chi-Tai
Chen, Chien-Ming
Yeh, Chuan-Feng
Li, Chi-Han
Lai, Gi-Ming
Yao, Chih-Jung
Yan, Jiann-Long
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Aberrant activation of Wnt/β-catenin signaling plays an important role in the development of colon cancer.
HS7 is an active fraction extracted from Taiwanofungus camphoratus, which had been widely used as complementary medicine for Taiwan cancer patients in the past decades.
In this study, we demonstrated the effects of HS7 on the growth inhibition, apoptosis induction, and Wnt/β-catenin signaling suppression in human colon cancer cells.
HS7 significantly inhibited proliferation of HT29, HCT116, and SW480 colon cancer cells in a dose- and time-dependent manner.
The apoptosis induction was evidenced by DNA fragmentation and subG1 accumulation, which was associated with increased Bax/Bcl-2 ratio, activation of caspase-3 and cleavage of PARP.
By using Tcf-dependent luciferase activity assay, HS7 was found to inhibit the β-catenin/Tcf transcriptional activities.
In addition, HS7 strongly suppressed the binding of Tcf complexes to its DNA-binding site shown in electrophoretic mobility shift assay.
This inhibition was further confirmed by the decreased protein levels of Tcf-4 and β-catenin.
The β-catenin/Tcf downstream target genes, such as survivin, c-myc, cyclin D1, MMP7, and MT1-MMP involved in apoptosis, invasion, and angiogenesis were also diminished as well.
These results indicate that Taiwanofungus camphoratus may provide a benefit as integrative medicine for the treatment of colon cancer.
American Psychological Association (APA)
Yeh, Chi-Tai& Yao, Chih-Jung& Yan, Jiann-Long& Chuang, Shuang-En& Lee, Liang-Ming& Chen, Chien-Ming…[et al.]. 2011. Apoptotic Cell Death and Inhibition of Wntβ-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-495742
Modern Language Association (MLA)
Yeh, Chi-Tai…[et al.]. Apoptotic Cell Death and Inhibition of Wntβ-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-495742
American Medical Association (AMA)
Yeh, Chi-Tai& Yao, Chih-Jung& Yan, Jiann-Long& Chuang, Shuang-En& Lee, Liang-Ming& Chen, Chien-Ming…[et al.]. Apoptotic Cell Death and Inhibition of Wntβ-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) from Taiwanofungus camphoratus. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-495742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495742