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Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions
Joint Authors
Yu, Zhongtang
Morrison, Mark
Stiverson, Jill
Source
International Journal of Microbiology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
The objective of this study was to assess the importance of select cultured and uncultured bacteria in the rumen by quantifying their populations and the effect of diets and ruminal fractions.
Full-length 16S rRNA gene (rrs) sequences were recovered from rumen samples using specific primers designed from partial sequences recovered previously.
Five uncultured bacterial operational taxonomic units (OTUs) were quantified using specific quantitative PCR (qPCR) in fractionated ruminal samples from sheep fed either hay alone or hay plus corn.
Species Fibrobacter succinogenes, Ruminococcus albus, R.
flavefaciens, Ruminobacter amylophilus, Selenomonas ruminantium, and Mitsuokella multacida and genera Butyrivibrio and Prevotella were also quantified as comparison.
The full-length rrs sequence improved taxonomic assignments of partial rrs sequences.
Genus Prevotella had the greatest abundance.
Of the three major cultured cellulolytic species, R.
flavefaciens was most abundant, followed by R.
albus and F.
succinogenes.
The five uncultured bacterial OTUs, classified to genus Acetivibrio, genus Allobaculum, family Ruminococcaceae, order Clostridiales, or class Clostridia, had abundance comparable to that of the above species of genera except Prevotella.
Corn supplementation and fractions affected distribution of the rumen bacteria, but to a limited extent.
When compared to the qPCR data, sequence frequencies in the rrs clone libraries tended to overestimate the abundance of the bacteria represented.
This study showed that abundance and population dynamics of uncultured bacteria can be quantified by specific qPCR, which complements the results of rrs clone libraries.
This study also revealed that some uncultured bacteria might be as important as some of the well-characterized bacteria in the rumen.
The approach used should be applicable to assess the abundance and potential importance of uncultured bacteria in other environments.
American Psychological Association (APA)
Stiverson, Jill& Morrison, Mark& Yu, Zhongtang. 2011. Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-495791
Modern Language Association (MLA)
Stiverson, Jill…[et al.]. Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions. International Journal of Microbiology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-495791
American Medical Association (AMA)
Stiverson, Jill& Morrison, Mark& Yu, Zhongtang. Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions. International Journal of Microbiology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-495791
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495791