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Thermal Aggregation of Recombinant Protective Antigen : Aggregate Morphology and Growth Rate
Joint Authors
Miller, Aline F.
Belton, Daniel J.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The thermal aggregation of the biopharmaceutical protein recombinant protective antigen (rPA) has been explored, and the associated kinetics and thermodynamic parameters have been extracted using optical and environmental scanning electron microscopies (ESEMs) and ultraviolet light scattering spectroscopy (UV-LSS).
Visual observations and turbidity measurements provided an overall picture of the aggregation process, suggesting a two-step mechanism.
Microscopy was used to examine the structure of aggregates, revealing an open morphology formed by the clustering of the microscopic aggregate particles.
UV-LSS was used and developed to elucidate the growth rate of these particles, which formed in the first stage of the aggregation process.
Their growth rate is observed to be high initially, before falling to converge on a final size that correlates with the ESEM data.
The results suggest that the particle growth rate is limited by rPA monomer concentration, and by obtaining data over a range of incubation temperatures, an approach was developed to model the aggregation kinetics and extract the rate constants and the temperature dependence of aggregation.
In doing so, we quantified the susceptibility of rPA aggregation under different temperature and environmental conditions and moreover demonstrated a novel use of UV spectrometry to monitor the particle aggregation quantitatively, in situ, in a nondestructive and time-resolved manner.
American Psychological Association (APA)
Belton, Daniel J.& Miller, Aline F.. 2013. Thermal Aggregation of Recombinant Protective Antigen : Aggregate Morphology and Growth Rate. Journal of Biophysics،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-495840
Modern Language Association (MLA)
Belton, Daniel J.& Miller, Aline F.. Thermal Aggregation of Recombinant Protective Antigen : Aggregate Morphology and Growth Rate. Journal of Biophysics No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-495840
American Medical Association (AMA)
Belton, Daniel J.& Miller, Aline F.. Thermal Aggregation of Recombinant Protective Antigen : Aggregate Morphology and Growth Rate. Journal of Biophysics. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-495840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495840