Biosynthesis and Immobilization of Biofunctional Allophycocyanin
Joint Authors
Chen, Huaxin
Li, Fuchao
Jiang, Peng
Cui, Yulin
Liu, Shaofang
Chen, Yingjie
Qin, Song
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli.
The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot.
Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles.
The holo-allophycocyanin-α subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles.
Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors.
Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.
American Psychological Association (APA)
Chen, Yingjie& Liu, Shaofang& Cui, Yulin& Jiang, Peng& Chen, Huaxin& Li, Fuchao…[et al.]. 2011. Biosynthesis and Immobilization of Biofunctional Allophycocyanin. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-495857
Modern Language Association (MLA)
Chen, Yingjie…[et al.]. Biosynthesis and Immobilization of Biofunctional Allophycocyanin. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-495857
American Medical Association (AMA)
Chen, Yingjie& Liu, Shaofang& Cui, Yulin& Jiang, Peng& Chen, Huaxin& Li, Fuchao…[et al.]. Biosynthesis and Immobilization of Biofunctional Allophycocyanin. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-495857
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495857