Anti-Inflammatory Activity of Lactobacillus on Carrageenan-Induced Paw Edema in Male Wistar Rats

Abstract EN

Introduction.

Lactobacillus casei and Lactobacillus acidophilus were used to assess the anti-inflammatory properties in carrageenan induced acute inflammatory model.

Materials and Methods.

Diclofenac sodium was used as standard drug at concentration of 150 mg/kg of body weight.

Culture of Lactobacillus 2×107 CFU/ml was given orally.

Edema was induced with 1% carrageenan to all the groups after one hour of the oral treatments.

Paw thickness was checked at t=1, 2, 3, 4, 5, and 24 hours.

Stair climbing score and motility score were assessed at t=24 hours.

Cytokines assay for IL-6, IL-10, and TNF-α was performed on serum samples.

Results.

Lactobacillus showed a statistically significant decrease in paw thickness at P<0.001.

L.

acidophilus and L.

casei decreased by 32% and 28% in paw thickness.

They both significantly increased the stair climbing and motility score.

Lactobacillus treatment significantly downregulated IL-6 and TNF-α while upregulated IL-10 at P<0.0001.

Conclusion.

L.

casei and L.

acidophilus significantly decreased the inflammatory reactions induced by carrageenan.

This study has also proposed that Lactobacillus ameliorated the inflammatory reaction by downregulating the proinflammatory cytokines pathway.