Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide from Sorghum bicolor L. Moench
Joint Authors
Sant, Rajnesh
Ahmad, Norazlina
Godwin, Ian D.
Steadman, Kathryn J.
Bokan, Milovan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Regulatory sequences with endosperm specificity are essential for foreign gene expression in the desired tissue for both grain quality improvement and molecular pharming.
In this study, promoters of seed storage α-kafirin genes coupled with signal sequence (ss) were isolated from Sorghum bicolor L.
Moench genomic DNA by PCR.
The α-kafirin promoter (α-kaf) contains endosperm specificity-determining motifs, prolamin-box, the O2-box 1, CATC, and TATA boxes required for α-kafirin gene expression in sorghum seeds.
The constructs pMB-Ubi-gfp and pMB-kaf-gfp were microprojectile bombarded into various sorghum and sweet corn explants.
GFP expression was detected on all explants using the Ubi promoter but only in seeds for the α-kaf promoter.
This shows that the α-kaf promoter isolated was functional and demonstrated seed-specific GFP expression.
The constructs pMB-Ubi-ss-gfp and pMB-kaf-ss-gfp were also bombarded into the same explants.
Detection of GFP expression showed that the signal peptide (SP)::GFP fusion can assemble and fold properly, preserving the fluorescent properties of GFP.
American Psychological Association (APA)
Ahmad, Norazlina& Sant, Rajnesh& Bokan, Milovan& Steadman, Kathryn J.& Godwin, Ian D.. 2012. Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide from Sorghum bicolor L. Moench. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-495938
Modern Language Association (MLA)
Ahmad, Norazlina…[et al.]. Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide from Sorghum bicolor L. Moench. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-495938
American Medical Association (AMA)
Ahmad, Norazlina& Sant, Rajnesh& Bokan, Milovan& Steadman, Kathryn J.& Godwin, Ian D.. Expression Pattern of the Alpha-Kafirin Promoter Coupled with a Signal Peptide from Sorghum bicolor L. Moench. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-495938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495938