An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids
Joint Authors
Mathieu-Daudé, Françoise
Garcia, Déborah
Sereno, Denis
Corrales, Rosa M.
Brenière, Simone F.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Extracellular factors produced by Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei are important in the host-parasite relationship.
Here, we describe a genome-based approach to identify putative extracellular proteins conserved among trypanosomatids that are likely involved in the classical secretory pathway.
Potentially secreted proteins were identified by bioinformatic analysis of the T.
cruzi genome.
A subset of thirteen genes encoding unknown proteins with orthologs containing a signal peptide sequence in L.
infantum, L.
major, and T.
brucei were transfected into L.
infantum.
Tagged proteins detected in the extracellular medium confirmed computer predictions in about 25% of the hits.
Secretion was confirmed for two L.
infantum orthologs proteins using the same experimental system.
Infectivity studies of transgenic Leishmania parasites suggest that one of the secreted proteins increases parasite replication inside macrophages.
This methodology can identify conserved secreted proteins involved in the classical secretory pathway, and they may represent potential virulence factors in trypanosomatids.
American Psychological Association (APA)
Corrales, Rosa M.& Mathieu-Daudé, Françoise& Garcia, Déborah& Brenière, Simone F.& Sereno, Denis. 2010. An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-495967
Modern Language Association (MLA)
Corrales, Rosa M.…[et al.]. An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-495967
American Medical Association (AMA)
Corrales, Rosa M.& Mathieu-Daudé, Françoise& Garcia, Déborah& Brenière, Simone F.& Sereno, Denis. An Experimental Approach for the Identification of Conserved Secreted Proteins in Trypanosomatids. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-495967
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495967