Isolation and Screening of Polyhydroxyalkanoates Producing Bacteria from Pulp, Paper, and Cardboard Industry Wastes
Joint Authors
Bhuwal, Anish Kumari
Aggarwal, Neeraj Kumar
Goyal, Varsha
Singh, Gulab
Yadav, Anita
Source
International Journal of Biomaterials
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Polyhydroxyalkanoates (PHAs) are storage materials that accumulate by various bacteria as energy and carbon reserve materials.
They are biodegradable, environmentally friendly, and also biocompatible bioplastics.
Unlike petrochemical-based plastics that take several decades to fully degrade, PHAs can be completely degraded within a year by variety of microorganisms into CO2 and water.
In the present study, we aim to utilize pulp, paper, and cardboard industry sludge and waste water for the isolation and screening of polyhydroxyalkanoates (PHAs) accumulating bacteria and production of cost-effective PHB using cardboard industry waste water.
Results.
A total of 42 isolates showed black-blue coloration when stained with Sudan black B, a preliminary screening agent for lipophilic compounds, and a total of 15 isolates showed positive result with Nile blue A staining, a more specific dye for PHA granules.
The isolates NAP11 and NAC1 showed maximum PHA production 79.27% and 77.63% with polymer concentration of 5.236 g/L and 4.042 g/L with cardboard industry waste water.
Both of the selected isolates, NAP11 and NAC1, were classified up to genus level by studying their morphological and biochemical characteristics and were found to be Enterococcus sp., Brevundimonas sp.
and, respectively.
Conclusion.
The isolates Enterococcus sp.
NAP11 and Brevundimonas sp.
NAC1 can be considered as good candidates for industrial production of PHB from cardboard industry waste water.
We are reporting for the first time the use of cardboard industry waste water as a cultivation medium for the PHB production.
American Psychological Association (APA)
Bhuwal, Anish Kumari& Singh, Gulab& Aggarwal, Neeraj Kumar& Goyal, Varsha& Yadav, Anita. 2013. Isolation and Screening of Polyhydroxyalkanoates Producing Bacteria from Pulp, Paper, and Cardboard Industry Wastes. International Journal of Biomaterials،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495980
Modern Language Association (MLA)
Bhuwal, Anish Kumari…[et al.]. Isolation and Screening of Polyhydroxyalkanoates Producing Bacteria from Pulp, Paper, and Cardboard Industry Wastes. International Journal of Biomaterials No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-495980
American Medical Association (AMA)
Bhuwal, Anish Kumari& Singh, Gulab& Aggarwal, Neeraj Kumar& Goyal, Varsha& Yadav, Anita. Isolation and Screening of Polyhydroxyalkanoates Producing Bacteria from Pulp, Paper, and Cardboard Industry Wastes. International Journal of Biomaterials. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-495980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-495980