Effect of Non-Anticoagulant N-Desulfated Heparin on Basic Fibroblast Growth Factor Expression, Angiogenesis, and Metastasis of Gastric Carcinoma In Vitro and In Vivo
Joint Authors
Dong, Ying
Chen, Ming-Xiang
Chen, Jin-Lian
Fan, Jing
Gu, Jian-Zhong
Source
Gastroenterology Research and Practice
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
The present study was performed to investigate the effect of N-desulfated heparin on basic fibroblast growth factor (bFGF) expression, tumor angiogenesis and metastasis of gastric carcinoma.
Methods.
Human gastric cancer SGC-7901 tissues were orthotopically implanted into the stomach of NOD SCID mice.
Twenty mice were randomly divided into two groups which received either intravenous injection of 0.9% NaCl solution (normal saline group) or 10 mg/kg N-desulfated heparin (N-desulfated heparin group) twice weekly for three weeks.
In vitro, human gastric carcinoma SGC-7901 cells were treated with N-desulfated heparin in different concentration (0.1 mg/mL, 1 mg/mL, N-desulfated heparin group), and treated with medium (control group).
Results.
In vivo, the tumor metastasis rates were 9/10 in normal saline group and 2/10 in N-desulfated heparin group (P<0.05).
The intratumoral microvessel density was higher in normal saline group than in N-desulfated heparin group (P<0.05).
bFGF expression in gastric tissue was inhibited by N-desulfated heparin (P<0.05).
There was no bleeding in N-desulfated heparin group.
In vitro, N-desulfated heparin inhibited significantly bFGF protein and mRNA expression of gastric carcinoma cells (P<0.05).
Conclusions.
N-desulfated heparin can inhibit the metastasis of gastric cancer through inhibiting tumor bFGF expression and tumor angiogenesis with no obvious anticoagulant activity.
American Psychological Association (APA)
Chen, Jin-Lian& Fan, Jing& Chen, Ming-Xiang& Dong, Ying& Gu, Jian-Zhong. 2012. Effect of Non-Anticoagulant N-Desulfated Heparin on Basic Fibroblast Growth Factor Expression, Angiogenesis, and Metastasis of Gastric Carcinoma In Vitro and In Vivo. Gastroenterology Research and Practice،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-496001
Modern Language Association (MLA)
Chen, Jin-Lian…[et al.]. Effect of Non-Anticoagulant N-Desulfated Heparin on Basic Fibroblast Growth Factor Expression, Angiogenesis, and Metastasis of Gastric Carcinoma In Vitro and In Vivo. Gastroenterology Research and Practice No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-496001
American Medical Association (AMA)
Chen, Jin-Lian& Fan, Jing& Chen, Ming-Xiang& Dong, Ying& Gu, Jian-Zhong. Effect of Non-Anticoagulant N-Desulfated Heparin on Basic Fibroblast Growth Factor Expression, Angiogenesis, and Metastasis of Gastric Carcinoma In Vitro and In Vivo. Gastroenterology Research and Practice. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-496001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496001