Growth Kinetics and Mechanistic Action of Reactive Oxygen Species Released by Silver Nanoparticles from Aspergillus niger on Escherichia coli
Joint Authors
Hiremath, Jyothi
ul-Haq, Manzoor
Mathew, Jasmine
Singh, Dattu
Rathod, Vandana
Singh, Ashish Kumar
Ninganagouda, Shivaraj
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Silver Nanoparticles (AgNPs), the real silver bullet, are known to have good antibacterial properties against pathogenic microorganisms.
In the present study AgNPs were prepared from extracellular filtrate of Aspergillus niger.
Characterization of AgNPs by UV-Vis spectrum reveals specific surface plasmon resonance at peak 416 nm; TEM photographs revealed the size of the AgNPs to be 20–55 nm.
Average diameter of the produced AgNPs was found to be 73 nm with a zeta potential that was −24 mV using Malvern Zetasizer.
SEM micrographs showed AgNPs to be spherical with smooth morphology.
EDS revealed the presence of pure metallic AgNPs along with carbon and oxygen signatures.
Of the different concentrations (0, 2.5, 5, 10, and 15 μg/mL) used 10 μg/mL were sufficient to inhibit 107 CFU/mL of E.
coli.
ROS production was measured using DCFH-DA method and the the free radical generation effect of AgNPs on bacterial growth inhibition was investigated by ESR spectroscopy.
This paper not only deals with the damage inflicted on microorganisms by AgNPs but also induces cell death through the production of ROS released by AgNPs and also growth kinetics of E.
coli supplemented with AgNPs produced by A.
niger.
American Psychological Association (APA)
Ninganagouda, Shivaraj& Rathod, Vandana& Singh, Dattu& Hiremath, Jyothi& Singh, Ashish Kumar& Mathew, Jasmine…[et al.]. 2014. Growth Kinetics and Mechanistic Action of Reactive Oxygen Species Released by Silver Nanoparticles from Aspergillus niger on Escherichia coli. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-496044
Modern Language Association (MLA)
Ninganagouda, Shivaraj…[et al.]. Growth Kinetics and Mechanistic Action of Reactive Oxygen Species Released by Silver Nanoparticles from Aspergillus niger on Escherichia coli. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-496044
American Medical Association (AMA)
Ninganagouda, Shivaraj& Rathod, Vandana& Singh, Dattu& Hiremath, Jyothi& Singh, Ashish Kumar& Mathew, Jasmine…[et al.]. Growth Kinetics and Mechanistic Action of Reactive Oxygen Species Released by Silver Nanoparticles from Aspergillus niger on Escherichia coli. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-496044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496044