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The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon : A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea
Joint Authors
Lozada-Chávez, Irma
Amils, Ricardo
Marín, Irma
Rodriguez, Nuria
Montoya, Lilia
Source
International Journal of Microbiology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-11
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Our goal was to examine the composition of methanogenic archaea (MA) and sulfate-reducing (SRP) and sulfur-oxidizing (SOP) prokaryotes in the extreme athalassohaline and particularly sulfate-rich sediment of Tirez Lagoon (Spain).
Thus, adenosine-5′-phosphosulfate (APS) reductase α (aprA) and methyl coenzyme M reductase α (mcrA) gene markers were amplified given that both enzymes are specific for SRP, SOP, and MA, respectively.
Anaerobic populations sampled at different depths in flooded and dry seasons from the anoxic sediment were compared qualitatively via denaturing gradient gel electrophoresis (DGGE) fingerprint analysis.
Phylogenetic analyses allowed the detection of SRP belonging to Desulfobacteraceae, Desulfohalobiaceae, and Peptococcaceae in ∂-proteobacteria and Firmicutes and SOP belonging to Chromatiales/Thiotrichales clade and Ectothiorhodospiraceae in γ-proteobacteria as well as MA belonging to methylotrophic species in Methanosarcinaceae and one hydrogenotrophic species in Methanomicrobiaceae.
We also estimated amino acid composition, GC content, and preferential codon usage for the AprA and McrA sequences from halophiles, nonhalophiles, and Tirez phylotypes.
Even though our results cannot be currently conclusive regarding the halotolerant strategies carried out by Tirez phylotypes, we discuss the possibility of a plausible “salt-in” signal in SRP and SOP as well as of a speculative complementary haloadaptation between salt-in and salt-out strategies in MA.
American Psychological Association (APA)
Montoya, Lilia& Lozada-Chávez, Irma& Amils, Ricardo& Rodriguez, Nuria& Marín, Irma. 2011. The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon : A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-22.
https://search.emarefa.net/detail/BIM-496083
Modern Language Association (MLA)
Montoya, Lilia…[et al.]. The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon : A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea. International Journal of Microbiology No. 2011 (2011), pp.1-22.
https://search.emarefa.net/detail/BIM-496083
American Medical Association (AMA)
Montoya, Lilia& Lozada-Chávez, Irma& Amils, Ricardo& Rodriguez, Nuria& Marín, Irma. The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon : A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea. International Journal of Microbiology. 2011. Vol. 2011, no. 2011, pp.1-22.
https://search.emarefa.net/detail/BIM-496083
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496083