Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester
Joint Authors
Sims, Ronald C.
Tramp, Cody
Miller, Charles D.
Ellis, Joshua T.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The microbial diversity and metabolic potential of a methanogenic consortium residing in a 3785-liter anaerobic digester, fed with wastewater algae, was analyzed using 454 pyrosequencing technology.
DNA was extracted from anaerobic sludge material and used in metagenomic analysis through PCR amplification of the methyl-coenzyme M reductase α subunit (mcrA) gene using primer sets ML, MCR, and ME.
The majority of annotated mcrA sequences were assigned taxonomically to the genera Methanosaeta in the order Methanosarcinales.
Methanogens from the genus Methanosaeta are obligate acetotrophs, suggesting this genus plays a dominant role in methane production from the analyzed fermentation sample.
Numerous analyzed sequences within the algae fed anaerobic digester were unclassified and could not be assigned taxonomically.
Relative amplicon frequencies were determined for each primer set to determine the utility of each in pyrosequencing.
Primer sets ML and MCR performed better quantitatively (representing the large majority of analyzed sequences) than primer set ME.
However, each of these primer sets was shown to provide a quantitatively unique community structure, and thus they are of equal importance in mcrA metagenomic analysis.
American Psychological Association (APA)
Ellis, Joshua T.& Tramp, Cody& Sims, Ronald C.& Miller, Charles D.. 2012. Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester. ISRN Microbiology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-496092
Modern Language Association (MLA)
Ellis, Joshua T.…[et al.]. Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester. ISRN Microbiology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-496092
American Medical Association (AMA)
Ellis, Joshua T.& Tramp, Cody& Sims, Ronald C.& Miller, Charles D.. Characterization of a Methanogenic Community within an Algal Fed Anaerobic Digester. ISRN Microbiology. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-496092
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496092