A restriction enzyme from escherichia coli purification and general properties
Other Title(s)
أنزيم مقيد من بكتريا القولون التنقية والخواص العامة
Joint Authors
Barakat, Nadiyah Tariq
al-Ubaydi, Maysem Muzaffar
Shakarah, Makram Diya Jafar
Source
Engineering and Technology Journal
Issue
Vol. 27, Issue 05 (30 Apr. 2009), pp.954-961, 8 p.
Publisher
Publication Date
2009-04-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract AR
تمت تنقية أنزيم مقيد من بكتريا القولون حوالي 40-مرة مع استرداد مقداره 3 %، و تضمنت خطوات التنقية ترسيب الأنزيم بواسطة كبريتات الامونيوم ثم استرجاعه بواسطة كروماتوغرافيا الترشيح الهلامي (خلال عمودي سيفادكس-100 و دي-سليلوز)، و تم اختبار الأنزيم من خلال قطعه لدنا العاثية لامبدا إلى ثلاثة حزم، كما وجد 5 % كربوهدرات مما يعني كونه بروتين سكري.
أخيرا، و مع مقارنة الأنزيم بأنزيمات مقيدة تجارية، فأن الأنزيم أثبت أنه أنزيم مقيد يعتمد في نشاطه على أيون المغنيسيوم.
Abstract EN
Abstract An endonuclease restriction enzyme has been purified from E.
coli about 40-fold with DNAse and RNAse recoveries of about 3%.
The purification steps included precipitation of the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 and DEAE-cellulose chromatography.
The purified endonuclease was able to break lambda DNA into three bands.
The enzyme has 5% of carbohydrate moiety which means it is a glycoprotein.
Lastly, the comparison with other commercial restriction endonucleases proves that this enzyme is a restriction enzyme with enzymic activity dependent on Mg2+-
American Psychological Association (APA)
Shakarah, Makram Diya Jafar& Barakat, Nadiyah Tariq& al-Ubaydi, Maysem Muzaffar. 2009. A restriction enzyme from escherichia coli purification and general properties. Engineering and Technology Journal،Vol. 27, no. 05, pp.954-961.
https://search.emarefa.net/detail/BIM-49617
Modern Language Association (MLA)
Shakarah, Makram Diya Jafar…[et al.]. A restriction enzyme from escherichia coli purification and general properties. Engineering and Technology Journal Vol. 27, no. 05 (2009), pp.954-961.
https://search.emarefa.net/detail/BIM-49617
American Medical Association (AMA)
Shakarah, Makram Diya Jafar& Barakat, Nadiyah Tariq& al-Ubaydi, Maysem Muzaffar. A restriction enzyme from escherichia coli purification and general properties. Engineering and Technology Journal. 2009. Vol. 27, no. 05, pp.954-961.
https://search.emarefa.net/detail/BIM-49617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 958-959
Record ID
BIM-49617
