![](/images/graphics-bg.png)
Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin
Joint Authors
Shawky, Sara E.
Ramadan, Nesrin K.
Salem, Maissa Y.
Mohamed, Afaf O.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Two simple, accurate, and sensitive methods were developed for the determination of netobimin in the presence of its degradation product.
Method (A) was an HPLC method, performed on C18 column using acetonitrile/methanol/0.01 M potassium dihydrogen phosphate (56 : 14 : 30 by volume) as a mobile phase with a flow rate of 0.5 mL/min.
Detection was performed at 254 nm.
Method (B) was a TLC method, using silica gel 60 F254 plates; the optimized mobile phase was toluene/methanol/chloroform/ammonium hydroxide (5 : 4 : 6 : 0.1 by volume).
The spots were scanned densitometrically at 346 nm.
Linearity ranges were 1–10 μg/mL for method (A) and 0.5–5 μg/band for method (B), and the mean percentage recoveries were 99.3±0.7% and 99.7±0.7% for methods (A) and (B), respectively.
The proposed methods were found to be specific for netobimin in the presence of up to 90% of its degradation product.
Statistical comparison between the results obtained by these methods and the manufacturer method was done, and no significance difference was obtained.
American Psychological Association (APA)
Ramadan, Nesrin K.& Mohamed, Afaf O.& Shawky, Sara E.& Salem, Maissa Y.. 2012. Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-496173
Modern Language Association (MLA)
Ramadan, Nesrin K.…[et al.]. Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-496173
American Medical Association (AMA)
Ramadan, Nesrin K.& Mohamed, Afaf O.& Shawky, Sara E.& Salem, Maissa Y.. Different Stability-Indicating Chromatographic Techniques for the Determination of Netobimin. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-496173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496173