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Redox Regulation of Nonmuscle Myosin Heavy Chain during Integrin Engagement
Joint Authors
Cozzi, Giacomo
Fiaschi, Tania
Chiarugi, Paola
Source
Journal of Signal Transduction
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
On the basis of our findings reporting that cell adhesion induces the generation of reactive oxygen species (ROS) after integrin engagement, we were interested in identifying redox-regulated proteins during this process.
Mass spectrometry analysis led us to identify nonmuscle myosin heavy chain (nmMHC) as a target of ROS.
Our results show that, while nmMHC is reduced in detached/rounded cells, it turns towards an oxidized state in adherent/spread cells due to the integrin-engaged ROS machinery.
The functional role of nmMHC redox regulation is suggested by the redox sensitivity of its association with actin, suggesting a role of nmMHC oxidation in cytoskeleton movement.
Analysis of muscle MHC (mMHC) redox state during muscle differentiation, a process linked to a great and stable decrease of ROS content, shows that the protein does not undergo a redox control.
Hence, we propose that the redox regulation of MHC in nonprofessional muscle cells is mandatory for actin binding during dynamic cytoskeleton rearrangement, but it is dispensable for static and highly organized cytoskeletal contractile architecture in differentiating myotubes.
American Psychological Association (APA)
Fiaschi, Tania& Cozzi, Giacomo& Chiarugi, Paola. 2011. Redox Regulation of Nonmuscle Myosin Heavy Chain during Integrin Engagement. Journal of Signal Transduction،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-496203
Modern Language Association (MLA)
Fiaschi, Tania…[et al.]. Redox Regulation of Nonmuscle Myosin Heavy Chain during Integrin Engagement. Journal of Signal Transduction No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-496203
American Medical Association (AMA)
Fiaschi, Tania& Cozzi, Giacomo& Chiarugi, Paola. Redox Regulation of Nonmuscle Myosin Heavy Chain during Integrin Engagement. Journal of Signal Transduction. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-496203
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496203