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Issues and Applications in Label-Free Quantitative Mass Spectrometry
Joint Authors
Witzmann, Frank A.
Lai, Xianyin
Wang, Lianshui
Source
International Journal of Proteomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-01-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
To address the challenges associated with differential expression proteomics, label-free mass spectrometric protein quantification methods have been developed as alternatives to array-based, gel-based, and stable isotope tag or label-based approaches.
In this paper, we focus on the issues associated with label-free methods that rely on quantitation based on peptide ion peak area measurement.
These issues include chromatographic alignment, peptide qualification for quantitation, and normalization.
In addressing these issues, we present various approaches, assembled in a recently developed label-free quantitative mass spectrometry platform, that overcome these difficulties and enable comprehensive, accurate, and reproducible protein quantitation in highly complex protein mixtures from experiments with many sample groups.
As examples of the utility of this approach, we present a variety of cases where the platform was applied successfully to assess differential protein expression or abundance in body fluids, in vitro nanotoxicology models, tissue proteomics in genetic knock-in mice, and cell membrane proteomics.
American Psychological Association (APA)
Lai, Xianyin& Wang, Lianshui& Witzmann, Frank A.. 2013. Issues and Applications in Label-Free Quantitative Mass Spectrometry. International Journal of Proteomics،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-496209
Modern Language Association (MLA)
Lai, Xianyin…[et al.]. Issues and Applications in Label-Free Quantitative Mass Spectrometry. International Journal of Proteomics No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-496209
American Medical Association (AMA)
Lai, Xianyin& Wang, Lianshui& Witzmann, Frank A.. Issues and Applications in Label-Free Quantitative Mass Spectrometry. International Journal of Proteomics. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-496209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496209