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Pcr assay in malaria diagnosis using filter paper samples from jazan region, saudi arabia
Joint Authors
al-Harithi, Said A.
Jamjoom, Manal B.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 3 (31 Dec. 2008), pp.393-707, 315 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-12-31
Country of Publication
Egypt
No. of Pages
315
Main Subjects
Topics
Abstract EN
PCR assay using designed primers was evaluated in detecting human malaria infection from whole blood and / or on Whatman® filter-paper compared to conventional microscopy.
Two DNA extraction methods were used for dried blood-spots ; QIAamp mini kit and methanol-fixation / heat-extraction.
A total of 118 cases were collected from 4 hospitals at Jazan district.
Microscopic examination showed positivity in 66 / 118 samples (56 %).
Thin films showed parasitaemia from 1 + to 4 +.
In PCR assay, 79 samples (70 %) were positive for the genus Plasmodium given 153 base pair PCR product.
All microscopy positive samples were PCR positive but PCR detected 13 cases missed by microscopy.
As for filter-paper spot samples, 68 samples (57.6 %) were PCR positive when DNA was extracted by QIAamp mini kit whereas 49 (41.5 %) by methanol-fixation/heat-extracion method.
PCR sensitivity decreased by using DNA extracted from filter-paper compared to microscopy and whole blood PCR.
But the DNA isolated from filter paper detected parasites in many microscopy negative samples.
American Psychological Association (APA)
al-Harithi, Said A.& Jamjoom, Manal B.. 2008. Pcr assay in malaria diagnosis using filter paper samples from jazan region, saudi arabia. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 3, pp.393-707.
https://search.emarefa.net/detail/BIM-49624
Modern Language Association (MLA)
al-Harithi, Said A.& Jamjoom, Manal B.. Pcr assay in malaria diagnosis using filter paper samples from jazan region, saudi arabia. Journal of the Egyptian Society of Parasitology Vol. 38, no. 3 (Dec. 2008), pp.393-707.
https://search.emarefa.net/detail/BIM-49624
American Medical Association (AMA)
al-Harithi, Said A.& Jamjoom, Manal B.. Pcr assay in malaria diagnosis using filter paper samples from jazan region, saudi arabia. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 3, pp.393-707.
https://search.emarefa.net/detail/BIM-49624
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 702-707
Record ID
BIM-49624