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DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7
Joint Authors
Chen, Zhan-fang
Li, Jing
Mo, Xiao-mei
Qi, Xin
Li, Yan-tuan
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Vanadium compounds were studied during recent years to be considered as a representative of a new class of nonplatinum metal anticancer agents in combination to its low toxicity.
Here, we found a vanadium compound Van-7 as an inhibitor of Topo I other than Topo II using topoisomerase-mediated supercoiled DNA relaxation assay.
Agarose gel electrophoresis and comet assay showed that Van-7 treatment did not produce cleavable complexes like HCPT, thereby suggesting that Topo I inhibition occurred upstream of the relegation step.
Further studies revealed that Van-7 inhibited Topo I DNA binding involved in its intercalating DNA.
Van-7 did not affect the catalytic activity of DNase I even up to100 μM.
Van-7 significantly suppressed the growth of cancer cell lines with IC50 at nanomolar concentrations and arrested cell cycle of A549 cells at G2/M phase.
All these results indicate that Van-7 is a potential selective Topo I inhibitor with anticancer activities as a kind of Topo I suppressor, not Topo I poison.
American Psychological Association (APA)
Mo, Xiao-mei& Chen, Zhan-fang& Qi, Xin& Li, Yan-tuan& Li, Jing. 2012. DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7. Bioinorganic Chemistry and Applications،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-496252
Modern Language Association (MLA)
Mo, Xiao-mei…[et al.]. DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7. Bioinorganic Chemistry and Applications No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-496252
American Medical Association (AMA)
Mo, Xiao-mei& Chen, Zhan-fang& Qi, Xin& Li, Yan-tuan& Li, Jing. DNA-Binding and Topoisomerase-I-Suppressing Activities of Novel Vanadium Compound Van-7. Bioinorganic Chemistry and Applications. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-496252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496252