Validation of a Diagnostic Microarray for Human Papillomavirus : Coverage of 102 Genotypes

Abstract EN

Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa.

Existing assays for genotyping human papillomavirus are restricted to a small number of types.

Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs.

This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.