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Effects of HIF-1α and HIF2α on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts
Joint Authors
Turley, Helen
Leek, Russell
Troy, Helen
Chung, Yuen-Li
Gatter, Kevin
Harris, Adrian L.
Biswas, Swethajit
Pezzella, Francesco
Stubbs, Marion
Raval, Raju R.
Griffiths, John R.
Li, Ji-liang
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In cultured clear-cell renal carcinoma (CCRCC) 786-0 cells transfected with HIF1α (HIF-1+), HIF-2α (HIF-2+), or empty vector (EV), no significant differences were observed in the growth rates in vitro, but when grown in vivo as xenografts HIF-2α significantly increased, and HIF-1α significantly decreased growth rates, compared to EV tumors.
Factors associated with proliferation were increased and factors associated with cell death were decreased in HIF-2+ tumors.
Metabolite profiles showed higher glucose and lower lactate and alanine levels in the HIF-2+ tumors whilst immunostaining demonstrated higher pyruvate dehydrogenase and lower pyruvate dehydrogenase kinase 1, compared to control tumors.
Taken together, these results suggest that overexpression of HIF-2α in CCRCC 786-0 tumors regulated growth both by maintaining a low level of glycolysis and by allowing more mitochondrial metabolism and tolerance to ROS induced DNA damage.
The growth profiles observed may be mediated by adaptive changes to a more oxidative phenotype.
American Psychological Association (APA)
Biswas, Swethajit& Troy, Helen& Leek, Russell& Chung, Yuen-Li& Li, Ji-liang& Raval, Raju R.…[et al.]. 2010. Effects of HIF-1α and HIF2α on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts. Journal of Oncology،Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-496377
Modern Language Association (MLA)
Biswas, Swethajit…[et al.]. Effects of HIF-1α and HIF2α on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts. Journal of Oncology No. 2010 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-496377
American Medical Association (AMA)
Biswas, Swethajit& Troy, Helen& Leek, Russell& Chung, Yuen-Li& Li, Ji-liang& Raval, Raju R.…[et al.]. Effects of HIF-1α and HIF2α on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-14.
https://search.emarefa.net/detail/BIM-496377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496377