Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines
Joint Authors
Chawla, Raman
Sharma, Rakesh Kumar
Sharma, Ashok
Tripathi, Rajendra Prasad
Singh, Pankaj Kumar
Kumar, Raj
Jain, Swatantra Kumar
Arora, Rajesh
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines.
REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells.
The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated group.
More inhibition (~33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2 h prior irradiation.
Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells.
REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells.
Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (~0.6) as compared with Hep3B cells (~1.2) in REC-2006 + radiation-treated group.
In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3.
American Psychological Association (APA)
Singh, Pankaj Kumar& Kumar, Raj& Sharma, Ashok& Arora, Rajesh& Chawla, Raman& Jain, Swatantra Kumar…[et al.]. 2011. Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-496418
Modern Language Association (MLA)
Singh, Pankaj Kumar…[et al.]. Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-496418
American Medical Association (AMA)
Singh, Pankaj Kumar& Kumar, Raj& Sharma, Ashok& Arora, Rajesh& Chawla, Raman& Jain, Swatantra Kumar…[et al.]. Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-496418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496418