Citrus bergamia Risso Elevates Intracellular Ca2+ in Human Vascular Endothelial Cells due to Release of Ca2+ from Primary Intracellular Stores
Joint Authors
Kim, Hyo-Keun
Min, Sun Seek
Han, Seung Ho
Kang, Purum
Moon, Hea Kyung
Lee, Jeong-Min
Seol, Geun Hee
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The purpose of the present study is to examine the effects of essential oil of Citrus bergamia Risso (bergamot, BEO) on intracellular Ca2+ in human umbilical vein endothelial cells.
Fura-2 fluorescence was used to examine changes in intracellular Ca2+ concentration [Ca2+]i .
In the presence of extracellular Ca2+, BEO increased [Ca2+]i , which was partially inhibited by a nonselective Ca2+ channel blocker La3+.
In Ca2+-free extracellular solutions, BEO increased [Ca2+]i in a concentration-dependent manner, suggesting that BEO mobilizes intracellular Ca2+.
BEO-induced [Ca2+]i increase was partially inhibited by a Ca2+-induced Ca2+ release inhibitor dantrolene, a phospholipase C inhibitor U73122, and an inositol 1,4,5-triphosphate (IP3)-gated Ca2+ channel blocker, 2-aminoethoxydiphenyl borane (2-APB).
BEO also increased [Ca2+]i in the presence of carbonyl cyanide m-chlorophenylhydrazone, an inhibitor of mitochondrial Ca2+ uptake.
In addition, store-operated Ca2+ entry (SOC) was potentiated by BEO.
These results suggest that BEO mobilizes Ca2+ from primary intracellular stores via Ca2+-induced and IP3-mediated Ca2+ release and affect promotion of Ca2+ influx, likely via an SOC mechanism.
American Psychological Association (APA)
Kang, Purum& Han, Seung Ho& Moon, Hea Kyung& Lee, Jeong-Min& Kim, Hyo-Keun& Min, Sun Seek…[et al.]. 2013. Citrus bergamia Risso Elevates Intracellular Ca2+ in Human Vascular Endothelial Cells due to Release of Ca2+ from Primary Intracellular Stores. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-496537
Modern Language Association (MLA)
Kang, Purum…[et al.]. Citrus bergamia Risso Elevates Intracellular Ca2+ in Human Vascular Endothelial Cells due to Release of Ca2+ from Primary Intracellular Stores. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-496537
American Medical Association (AMA)
Kang, Purum& Han, Seung Ho& Moon, Hea Kyung& Lee, Jeong-Min& Kim, Hyo-Keun& Min, Sun Seek…[et al.]. Citrus bergamia Risso Elevates Intracellular Ca2+ in Human Vascular Endothelial Cells due to Release of Ca2+ from Primary Intracellular Stores. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-496537
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496537