Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAPDAHC Matrix
Joint Authors
Hou, Junjie
Cui, Ziyou
Xie, Zhensheng
Cai, Tanxi
Xue, Peng
Wu, Peng
Chen, Xiulan
Li, Jing
Yang, Fuquan
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Selecting an appropriate matrix solution is one of the most effective means of increasing the ionization efficiency of phosphopeptides in matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
In this study, we systematically assessed matrix combinations of 2, 6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC), and demonstrated that the low ratio DHAP/DAHC matrix was more effective in enhancing the ionization of phosphopeptides.
Low femtomole level of phosphopeptides from the tryptic digests of α-casein and β-casein was readily detected by MALDI-TOF-MS in both positive and negative ion mode without desalination or phosphopeptide enrichment.
Compared with the DHB/PA matrix, the optimized DHAP/DAHC matrix yielded superior sample homogeneity and higher phosphopeptide measurement sensitivity, particularly when multiple phosphorylated peptides were assessed.
Finally, the DHAP/DAHC matrix was applied to identify phosphorylation sites from α-casein and β-casein and to characterize two phosphorylation sites from the human histone H1 treated with Cyclin-Dependent Kinase-1 (CDK1) by MALDI-TOF/TOF MS.
American Psychological Association (APA)
Hou, Junjie& Xie, Zhensheng& Xue, Peng& Cui, Ziyou& Chen, Xiulan& Li, Jing…[et al.]. 2010. Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAPDAHC Matrix. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-496544
Modern Language Association (MLA)
Hou, Junjie…[et al.]. Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAPDAHC Matrix. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-496544
American Medical Association (AMA)
Hou, Junjie& Xie, Zhensheng& Xue, Peng& Cui, Ziyou& Chen, Xiulan& Li, Jing…[et al.]. Enhanced MALDI-TOF MS Analysis of Phosphopeptides Using an Optimized DHAPDAHC Matrix. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-496544
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496544