Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis
Joint Authors
Tavares, Ludgero C.
Simões, Marta
Carvalho, Rui A.
Mendes, Cândida
Grazina, Manuela M.
Botelho, Maria Filomena
Pires, Salomé
Abrantes, Ana M.
Casalta-Lopes, João
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
13C NMR isotopomer analysis was used to characterize intermediary metabolism in three colorectal cancer cell lines (WiDr, LS1034, and C2BBe1) and determine the “metabolic remodeling” that occurs under hypoxia.
Under normoxia, the three colorectal cancer cell lines present high rates of lactate production and can be seen as “Warburg” like cancer cells independently of substrate availability, since such profile was dominant at both high and low glucose media contents.
The LS1034 was the less glycolytic of the three cell lines and was the most affected by the event of hypoxia, raising abruptly glucose consumption and lactate production.
The other two colorectal cell lines, WiDr and C2BBe1, adapted better to hypoxia and were able to maintain their oxidative fluxes even at the very low levels of oxygen.
These differential metabolic behaviors of the three colorectal cell lines show how important an adequate knowledge of the “metabolic remodeling” that follows a given cancer treatment is towards the correct (re)design of therapeutic strategies against cancer.
American Psychological Association (APA)
Abrantes, Ana M.& Tavares, Ludgero C.& Pires, Salomé& Casalta-Lopes, João& Mendes, Cândida& Simões, Marta…[et al.]. 2014. Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-496554
Modern Language Association (MLA)
Abrantes, Ana M.…[et al.]. Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-496554
American Medical Association (AMA)
Abrantes, Ana M.& Tavares, Ludgero C.& Pires, Salomé& Casalta-Lopes, João& Mendes, Cândida& Simões, Marta…[et al.]. Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-496554
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496554