Silencing of Tumor Necrosis Factor Receptor 1 by siRNA in EC109 Cells Affects Cell Proliferation and Apoptosis
Joint Authors
Xiancai, Chen
Tianzhong, Ma
Zhongjing, Su
Ling, Chen
Changhui, Ma
Haibin, Chen
Ning, Wang
Ningxia, Zhu
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Tumor necrosis factor receptor 1 (TNFR1) is a membrane receptor able to bind TNF-α or TNF-β.
TNFR1 can suppress apoptosis by activating the NF-κB or JNK/SAPK signal transduction pathway, or it can induce apoptosis through a series of caspase cascade reactions; the particular effect may depend on the cell line.
In the present study, we first showed that TNFR1 is expressed at both the gene and protein levels in the esophageal carcinoma cell line EC109.
Then, by applying a specific siRNA, we silenced the expression of TNFR1; this resulted in a significant time-dependent promotion of cell proliferation and downregulation of the apoptotic rate.
These results suggest that TNFR1 is strongly expressed in the EC109 cell line and that it may play an apoptosis-mediating role, which may be suppressed by highly activated NF-κB.
American Psychological Association (APA)
Changhui, Ma& Tianzhong, Ma& Zhongjing, Su& Ling, Chen& Ning, Wang& Ningxia, Zhu…[et al.]. 2009. Silencing of Tumor Necrosis Factor Receptor 1 by siRNA in EC109 Cells Affects Cell Proliferation and Apoptosis. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-496610
Modern Language Association (MLA)
Changhui, Ma…[et al.]. Silencing of Tumor Necrosis Factor Receptor 1 by siRNA in EC109 Cells Affects Cell Proliferation and Apoptosis. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-496610
American Medical Association (AMA)
Changhui, Ma& Tianzhong, Ma& Zhongjing, Su& Ling, Chen& Ning, Wang& Ningxia, Zhu…[et al.]. Silencing of Tumor Necrosis Factor Receptor 1 by siRNA in EC109 Cells Affects Cell Proliferation and Apoptosis. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-496610
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496610