Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
Joint Authors
Kahl, Günter
Ferreira Neto, José Ribamar Costa
Benko-Iseppon, Ana Maria
Guimarães, Ana Carolina Ribeiro
Silva, Roberta Lane de Oliveira
Kido, Éderson Akio
Chabregas, Sabrina Moutinho
Veiga, Daniela Truffi
da Silva, Manassés Daniel
Burnquist, William Lee
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes.
Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified.
Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively.
At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding.
From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions.
Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed.
The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.
American Psychological Association (APA)
da Silva, Manassés Daniel& Silva, Roberta Lane de Oliveira& Ferreira Neto, José Ribamar Costa& Guimarães, Ana Carolina Ribeiro& Veiga, Daniela Truffi& Chabregas, Sabrina Moutinho…[et al.]. 2013. Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit. Journal of Nucleic Acids،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-496935
Modern Language Association (MLA)
da Silva, Manassés Daniel…[et al.]. Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit. Journal of Nucleic Acids No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-496935
American Medical Association (AMA)
da Silva, Manassés Daniel& Silva, Roberta Lane de Oliveira& Ferreira Neto, José Ribamar Costa& Guimarães, Ana Carolina Ribeiro& Veiga, Daniela Truffi& Chabregas, Sabrina Moutinho…[et al.]. Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit. Journal of Nucleic Acids. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-496935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496935