Altered Sympathetic-to-Immune Cell Signaling via β2-Adrenergic Receptors in Adjuvant Arthritis
Joint Authors
Lubahn, Cheri
Osredkar, Tracy
Schaller, Jill A.
Shewmaker, Eric
Lorton, Dianne
Bellinger, Denise L.
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-01
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Adjuvant-induced arthritic (AA) differentially affects norepinephrine concentrations in immune organs, and in vivo β-adrenergic receptor (β-AR) agonist treatment distinctly regulates ex vivo cytokine profiles in different immune organs.
We examined the contribution of altered β-AR functioning in AA to understand these disparate findings.
Twenty-one or 28 days after disease induction, we examined β2-AR expression in spleen and draining lymph nodes (DLNs) for the arthritic limbs using radioligand binding and western blots and splenocyte β-AR-stimulated cAMP production using enzyme-linked immunoassay (EIA).
During severe disease, β-AR agonists failed to induce splenocyte cAMP production, and β-AR affinity and density declined, indicating receptor desensitization and downregulation.
Splenocyte β2-AR phosphorylation (pβ2-AR) by protein kinase A (pβ2-ARPKA) decreased in severe disease, and pβ2-AR by G protein-coupled receptor kinases (pβ2-ARGRK) increased in chronic disease.
Conversely, in DLN cells, pβ2-ARPKA rose during severe disease, but fell during chronic disease, and pβ2-ARGRK increased during both disease stages.
A similar pβ2-AR pattern in DLN cells with the mycobacterial cell wall component of complete Freund’s adjuvant suggests that pattern recognition receptors (i.e., toll-like receptors) are important for DLN pβ2-AR patterns.
Collectively, our findings indicate lymphoid organ- and disease stage-specific sympathetic dysregulation, possibly explaining immune compartment-specific differences in β2-AR-mediated regulation of cytokine production in AA and rheumatoid arthritis.
American Psychological Association (APA)
Lorton, Dianne& Bellinger, Denise L.& Schaller, Jill A.& Shewmaker, Eric& Osredkar, Tracy& Lubahn, Cheri. 2013. Altered Sympathetic-to-Immune Cell Signaling via β2-Adrenergic Receptors in Adjuvant Arthritis. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-496983
Modern Language Association (MLA)
Lorton, Dianne…[et al.]. Altered Sympathetic-to-Immune Cell Signaling via β2-Adrenergic Receptors in Adjuvant Arthritis. Clinical and Developmental Immunology No. 2013 (2013), pp.1-17.
https://search.emarefa.net/detail/BIM-496983
American Medical Association (AMA)
Lorton, Dianne& Bellinger, Denise L.& Schaller, Jill A.& Shewmaker, Eric& Osredkar, Tracy& Lubahn, Cheri. Altered Sympathetic-to-Immune Cell Signaling via β2-Adrenergic Receptors in Adjuvant Arthritis. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-17.
https://search.emarefa.net/detail/BIM-496983
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496983