Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals
Joint Authors
Cui, Haixin
Li, Kui
Sun, Changjiao
Du, Wei
Wang, Yan
Cui, Jinhui
Ren, Hongyan
Source
Advances in Materials Science and Engineering
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Engineering Sciences and Information Technology
Abstract EN
To evaluate the performance of the magnetic nanoparticles as gene transfer vector for breeding transgenic animals, we investigated a new approach to deliver green fluorescent protein (GFP) gene to porcine kidney 15 (PK-15) and porcine embryonic fibroblast (PEF) cells using PEI-modified magnetic nanoparticles as gene vector.
The morphology of the nanoparticles and nanoparticle/DNA complexes was characterized using scanning electron microscopy.
It was found that the surface of the particles becomes coarse and rough with increased average diameter, which implied the effective conjugating between nanoparticles with DNA.
The zeta potential of nanoparticle/DNA complexes drops down from +29.4 mV to +23.1 mV comparing with pure nanoparticles.
Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease.
The efficiency of gene delivery was affected by the mass ratio of nanoparticle/DNA and the amount of nanoparticle/DNA complexes.
We confirm that the most optimal mass ratio of nanoparticle/DNA is 1 : 1 by conducting a series of experiments.
This work provides important experimental basis for the application of the magnetic nanoparticles on gene delivery to porcine somatic cells, which is significant for the achieving of breeding new transgenic cloned pigs by using somatic cell nuclear transfer technique.
American Psychological Association (APA)
Cui, Jinhui& Cui, Haixin& Wang, Yan& Sun, Changjiao& Li, Kui& Ren, Hongyan…[et al.]. 2012. Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals. Advances in Materials Science and Engineering،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-496990
Modern Language Association (MLA)
Cui, Jinhui…[et al.]. Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals. Advances in Materials Science and Engineering No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-496990
American Medical Association (AMA)
Cui, Jinhui& Cui, Haixin& Wang, Yan& Sun, Changjiao& Li, Kui& Ren, Hongyan…[et al.]. Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals. Advances in Materials Science and Engineering. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-496990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-496990