![](/images/graphics-bg.png)
Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications
Joint Authors
Kumpatla, Siva P.
Molina, Joe J.
Buenrostro-Nava, Marco T.
Beremand, Phillip D.
Riedel, Beth
Mirkov, T. Erik
Damaj, Mona B.
Thomas, Terry L.
Source
International Journal of Plant Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
High-throughput functional genomic procedures depend on the quality of the RNA used.
Copurifying molecules can negatively impact the functionality of some plant RNA preparations employed in these procedures.
We present a simplified, rapid, and scalable SDS/phenol-based method that provides the high-quantity and -quality RNA required by the newly emerging biotechnology applications.
The method is applied to isolating RNA from tissues of two biotechnologically important crop plants, sugarcane and citrus, which provide a challenge due to the presence of fiber, polysaccharides, or secondary metabolites.
The RNA isolated by this method is suitable for several downstream applications including northern blot hybridization, microarray analysis, and quantitative RT-PCR.
This method has been used in a diverse range of projects ranging from screening plant lines overexpressing mammalian genes to analyzing plant responses to viral infection and defense signaling molecules.
American Psychological Association (APA)
Damaj, Mona B.& Beremand, Phillip D.& Buenrostro-Nava, Marco T.& Riedel, Beth& Molina, Joe J.& Kumpatla, Siva P.…[et al.]. 2010. Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications. International Journal of Plant Genomics،Vol. 2009, no. 2009, pp.1-13.
https://search.emarefa.net/detail/BIM-497059
Modern Language Association (MLA)
Damaj, Mona B.…[et al.]. Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications. International Journal of Plant Genomics No. 2009 (2009), pp.1-13.
https://search.emarefa.net/detail/BIM-497059
American Medical Association (AMA)
Damaj, Mona B.& Beremand, Phillip D.& Buenrostro-Nava, Marco T.& Riedel, Beth& Molina, Joe J.& Kumpatla, Siva P.…[et al.]. Reproducible RNA Preparation from Sugarcane and Citrus for Functional Genomic Applications. International Journal of Plant Genomics. 2010. Vol. 2009, no. 2009, pp.1-13.
https://search.emarefa.net/detail/BIM-497059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497059