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Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products
Joint Authors
Frace, Michael A.
Mijatovic-Rustempasic, Slavica
Bowen, Michael D.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-23
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
The quality of sequencing results depends greatly upon the quality and purity of the template as well as the purity of the fluorescently labeled products generated by cycle sequencing.
Numerous approaches have been used for purification of cycle sequencing products, including alcohol precipitation, affinity-based chromatography, size exclusion chromatography, commercially-available proprietary methods, and paramagnetic bead technology.
In this paper, we describe an affordable paramagnetic technology method using BioMag Carboxyl beads.
Compared to other well-established, proprietary methods for purification of cycle sequencing products, this method produced consistently good results, with a very low reagent cost and short procedure time.
American Psychological Association (APA)
Mijatovic-Rustempasic, Slavica& Frace, Michael A.& Bowen, Michael D.. 2012. Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products. Sequencing،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-497191
Modern Language Association (MLA)
Mijatovic-Rustempasic, Slavica…[et al.]. Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products. Sequencing No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-497191
American Medical Association (AMA)
Mijatovic-Rustempasic, Slavica& Frace, Michael A.& Bowen, Michael D.. Cost-Effective Paramagnetic Bead Technique for Purification of Cycle Sequencing Products. Sequencing. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-497191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497191